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Artificial enzyme-based catalytic sensor for the electrochemical detection of 5-hydroxyindole-3-acetic acid tumor marker in urine
dc.contributor.author | Antuña Jiménez, Daniel | |
dc.contributor.author | Blanco López, María del Carmen | |
dc.contributor.author | Miranda Ordieres, Arturo José | |
dc.contributor.author | Lobo Castañón, María Jesús | |
dc.date.accessioned | 2015-08-20T06:57:10Z | |
dc.date.available | 2015-08-20T06:57:10Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Sensors and Actuators B:Chemical, 220, p. 688-694 (2015); doi:10.1016/j.snb.2015.05.109 | |
dc.identifier.issn | 0925-4005 | |
dc.identifier.uri | http://hdl.handle.net/10651/32535 | |
dc.description.abstract | A novel approach towards the development of biomimetic electrochemical sensors is presented. It is basedon magnetic-core/porous-shell molecularly imprinted composites as recognition phase. The selected sub-strate is 5-hydroxyindole-3-acetic acid (5-HIAA), an urinary metabolite used as carcinoid tumor maker.Using 5-HIAA as template molecule, hemin, 4-vinylpyridine and ethylenglycoldimethacrylate crosslinkerare polymerized onto the magnetic core to obtain an artificial enzyme with peroxidase activity. An aliquotof a suspension of the resulting material is deposited onto a carbon screen printed electrode, and the solidmaterial is fixed with a permanent magnet to prepare the sensor. The product of the catalytic oxidation of5-HIAA at the sensing phase is monitored by differential pulse voltammetry. This sensor allows the detec-tion of 5-HIAA with good sensitivity (0.72 ± 0.01 nA M−1) and linear response over a concentration rangecovering the whole window of normal and pathologic urinary levels of 5-HIAA 1–50 M (0.2–9.6 ppm)with a detection limit of 1.4 M (0.27 ppm). Urine samples of healthy individuals, spiked with knownconcentrations of 5-HIAA, yield good recovery values and reproducibility for normal and pathologicalconcentrations (90–100% recovery values, with RSD below 10%). | spa |
dc.description.sponsorship | D. Antuña-Jiménez thanks Principado de Asturias Government FICYT foundation for a Ph.D. Grant (BP09059). This work was finan-cially supported by the Spanish Government, Ministerio de Ciencia y Tecnología (CTQ2008-02429/BQU) and the European Social Fund | |
dc.format.extent | p. 688-694 | spa |
dc.language.iso | eng | spa |
dc.publisher | Elsevier | |
dc.relation.ispartof | Sensors and Actuators B: Chemical 220 | spa |
dc.rights | © 2015 Elsevier B.V. | |
dc.title | Artificial enzyme-based catalytic sensor for the electrochemical detection of 5-hydroxyindole-3-acetic acid tumor marker in urine | spa |
dc.type | journal article | |
dc.identifier.doi | 10.1016/j.snb.2015.05.109 | |
dc.relation.projectID | CTQ2008-02429/BQU | |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.snb.2015.05.109 |
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